Dilution Calculator
Dilution Calculator
Selected Dilution:
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Serum Quantity:
0
µL
Dilution Solution:
0
µL
Final Result:
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When Do We Perform Dilution in Laboratory Testing?
Dilution is performed when a patient sample is too concentrated or when the test value is higher than the analyzer measuring range.
1. Result Above Linear Range
When the analyzer shows:
- Above Range
- High
- Out of Range
- Greater Than Maximum Limit
The sample must be diluted and tested again.
Example:
Glucose > 600 mg/dL
Creatinine Very High
Glucose > 600 mg/dL
Creatinine Very High
2. Highly Concentrated Sample
If the serum contains very high analyte concentration, the reagent reaction may become inaccurate.
Dilution reduces concentration and helps obtain accurate results.
3. Instrument Limitation
Every analyzer has a maximum measuring range.
Analyzer Range = 0–300 mg/dL
Patient Result = 650 mg/dL
Patient Result = 650 mg/dL
The sample must be diluted before rerunning the test.
Common Dilution Ratios
| Dilution | Serum | Diluent |
|---|---|---|
| 1:2 | 100 µL | 100 µL |
| 1:5 | 100 µL | 400 µL |
| 1:10 | 100 µL | 900 µL |
| 1:20 | 100 µL | 1900 µL |
Final Result Formula
Final Result = Analyzer Reading × Dilution Factor
Example:
Dilution = 1:10
Analyzer Reading = 120 mg/dL
120 × 10 = 1200 mg/dL
Dilution = 1:10
Analyzer Reading = 120 mg/dL
120 × 10 = 1200 mg/dL
Tests Commonly Requiring Dilution
- Glucose
- Urea
- Creatinine
- Bilirubin
- CRP
- Ferritin
- Triglycerides
- HbA1c
- Hormone Assays
Important Points
- Always apply the dilution factor to the final result
- Proper mixing is essential
- Incorrect dilution can produce wrong patient results
- Re-run testing after dilution is recommended
