Estimation of Blood Glucose

 

Aim : Estimation of Blood Glucose

Requirements :

  •  Specimen :
Serum or Plasma free from haemolysis. Sodium Fluoride tube is preferred.

  • Reagents: 

    1. Glucose Standard (100 mg/dl)
    2. GOSD-POD reagent Enzyme reagent mixture containing Glucose Oxidase (GOD), Peroxidase (POD).
  • Instruments:
    1. Test tubes
    2. Pipettes, disposable tips, rack
    3. Water bath
    4. Colorimeter
Procedure:
    1. Labeled three clean and dry test tubes as Blank(B), Standard(S) and Test(T).
    2. Pipetting as following:
Test Blank Standard Test
GOD-POD Reagent 1 ml 1 ml 1 ml
Distilled water 10 ul - -
Glucose Standard - 10 ul -
Sample - - 10 ul
    1. Mix well and incubate at 37℃ for 10 minutes. or at room temperature 25℃ for 30 minutes.
    2. Measure at 540nm (Green filter) against blank within 60 minutes.
Calculation:
    Calculate the concentration of blood glucose in the specimen using the following formula:

Normal Range :
  • Blood :
    • Random Blood Sugar : <140 mg/dl
    • Fasting Blood Sugar : 70 - 110 mg/dl
    • Post Parendial Blood Sugar : <140 mg/dl
  • Urine :
    • Urine Blood Sugar : Absent or Nil
Critical Value :
  • Blood Glucose : >500 mg/dl

Popular Posts